The Stars fell four runs short of the Renegades’ 16 run total in the Super Over to lose the derby clash.
The Stars were sent in first in sunny conditions and when Elyse Villani fell for 17, responsibility fell to captain Meg Lanning and Mignon du Preez to take control of the innings.
The pair put on 52 runs together before Lanning (41) was trapped LBW by former Star Makinley Blows.
A quickfire 24 from Nat Sciver kept the Stars moving but it was Renegades spinner Ella Hayward who caused the damage.
Hayward took the wickets of Sciver (24), du Preez (40), Osborne (2) and Flintoff (2) to have the Stars batting depth truly tested for the first time.
With the Stars at 7-134 in the 18th over, in came Alana King who smashed 27 off just 12 balls to drive the Stars to a competitive 8-165 total.
Needing a strong response with the ball, Holly Ferling dismissed Sophie Molineux in the second over before Sophie Day took the key wicket of another former Star Lizelle Lee for 28.
The Stars had the Renegades well placed at 3-45 in the sixth over but it was Player of the Match Courtney Webb who changed the game for the Renegades, belting 54* from just 33 balls to push the Gades to equal the mark at 165.
After big hitting from Lizelle Lee set the Stars 16 to win the Super Over, Lanning and du Preez could only find 12 runs in reply.
The Stars are next in action on Tuesday afternoon against the Perth Scorchers at Hurstville